Nanomechanical forces generated by surface grafted DNA

被引:127
作者
Hagan, MF
Majumdar, A
Chakraborty, AK [1 ]
机构
[1] Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Mech Engn, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[4] Lawrence Berkeley Natl Lab, Div Mat Sci, Berkeley, CA 94720 USA
[5] Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA
关键词
D O I
10.1021/jp020972o
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Recent experiments show that the adsorption of biomolecules on one surface of a microcantilever generates surface stresses that cause the cantilever to deflect. If a second species binds to the adsorbed molecules, the stresses change, resulting in a different deflection. By choosing adsorbed probe molecules that recognize specific molecules, it may be possible to detect pathogens and biohazards. In particular, Fritz et al. (Fritz, J.; Baller, M. K.; Lang, H. P.; Rothuizen, H.; Vettiger, P.; Meyer, E.; Guntherodt, H.-J.; Gerber, Ch.; Gimzewski, J. K. Science 2000, 288, 316) and Wu et al. (Wu, G.; Haifeng, J.; Hansen, K.; Thundat, T.; Datar, R.; Cote, R.; Hagan, M. F.; Chakraborty, A. K.; Majumdar, A. Proc. Natl. Acad. Sci. U.S.A. 2001, 98, 1560) show that the presence of an individual sequence of DNA may be identified by observing the change in deflection as hybridization occurs. Also, it has been shown that this platform can detect prostate specific antigen (PSA). However, to exploit this phenomenon for the development of reliable microdevices, it is necessary to understand the origin of the nanomechanical forces that lead to cantilever deflection upon molecular recognition, as well as the dependence of such deflections on the identity and concentration of the target molecule. In this paper, we present a model with which we examine cantilever deflections resulting from adsorption and subsequent hybridization of DNA molecules. Using an empirical potential, we predict deflections upon hybridization that are consistent with experimental results. We find that the dominant contribution to these deflections arises from hydration forces, not conformational entropy or electrostatics. Cantilever deflections upon adsorption of single stranded DNA are smaller that those predicted after hybridization for reasonable interaction strengths. This is consistent with results in Fritz et al., but not those in Wit et al. The deflections predicted for DNA before and after hybridization are strongly dependent on surface coverage, as well as the degree of disorder on the surface. We argue that self-assembly of probe molecules on the cantilever surface must be carefully controlled and characterized for the realization of microdevices for pathogen detection that rely on nanomechanical forces generated by molecular recognition.
引用
收藏
页码:10163 / 10173
页数:11
相关论文
共 50 条
  • [41] Selective desorption of alkanethiols in mixed self-assembled monolayers for subsequent oligonucleotide attachment and DNA hybridization
    Satjapipat, M
    Sanedrin, R
    Zhou, FM
    [J]. LANGMUIR, 2001, 17 (24) : 7637 - 7644
  • [42] Overstretching B-DNA: The elastic response of individual double-stranded and single-stranded DNA molecules
    Smith, SB
    Cui, YJ
    Bustamante, C
    [J]. SCIENCE, 1996, 271 (5250) : 795 - 799
  • [43] Immobilization of nucleic acids at solid surfaces: Effect of oligonucleotide length on layer assembly
    Steel, AB
    Levicky, RL
    Herne, TM
    Tarlov, MJ
    [J]. BIOPHYSICAL JOURNAL, 2000, 79 (02) : 975 - 981
  • [44] Equation of state for polymer liquid crystals: Theory and experiment
    Strey, HH
    Parsegian, VA
    Podgornik, R
    [J]. PHYSICAL REVIEW E, 1999, 59 (01) : 999 - 1008
  • [45] Equation of state for DNA liquid crystals: Fluctuation enhanced electrostatic double layer repulsion
    Strey, HH
    Parsegian, VA
    Podgornik, R
    [J]. PHYSICAL REVIEW LETTERS, 1997, 78 (05) : 895 - 898
  • [46] Persistence length of single-stranded DNA
    Tinland, B
    Pluen, A
    Sturm, J
    Weill, G
    [J]. MACROMOLECULES, 1997, 30 (19) : 5763 - 5765
  • [47] CHAIN STIFFNESS AND BOND CORRELATIONS IN POLYMER BRUSHES
    WIJMANS, CM
    LEERMAKERS, FAM
    FLEER, GJ
    [J]. JOURNAL OF CHEMICAL PHYSICS, 1994, 101 (09) : 8214 - 8223
  • [48] Bioassay of prostate-specific antigen (PSA) using microcantilevers
    Wu, GH
    Datar, RH
    Hansen, KM
    Thundat, T
    Cote, RJ
    Majumdar, A
    [J]. NATURE BIOTECHNOLOGY, 2001, 19 (09) : 856 - 860
  • [49] Origin of nanomechanical cantilever motion generated from biomolecular interactions
    Wu, GH
    Ji, HF
    Hansen, K
    Thundat, T
    Datar, R
    Cote, R
    Hagan, MF
    Chakraborty, AK
    Majumdar, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (04) : 1560 - 1564
  • [50] Modelling of the orientational and structural dynamics of single-stranded DNA probe molecules immobilized on fused silica and glass surfaces
    Zeng, JA
    Wang, XF
    Krull, UJ
    [J]. INTERNATIONAL CONFERENCE ON SENSOR TECHNOLOGY (ISTC 2001), PROCEEDINGS, 2001, 4414 : 1 - 7