Cloning and characterization of Chinese hamster p53 cDNA

被引：70

作者：

Lee, HY

Larner, JM

Hamlin, JL

机构：

[1] UNIV VIRGINIA, SCH MED, DEPT BIOCHEM, CHARLOTTESVILLE, VA 22908 USA

[2] UNIV VIRGINIA, SCH MED, DEPT RADIAT ONCOL, CHARLOTTESVILLE, VA 22908 USA

来源：

GENE | 1997年 / 184卷 / 02期

关键词：

anti-oncogene; polymerase chain reaction; G(1) arrest;

D O I：

10.1016/S0378-1119(96)00592-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned and sequenced Chinese hamster p53 cDNA and have compared the p53 sequence in different Chinese hamster cell lines to several relevant phenotypes. Our results indicate that a mutation in CHO cells that changes Thr(211) to Lys(211) abrogates the ability to arrest in G(1) and apparently renders cells capable of amplifying DNA. However, this mutation has no effect on the G(2) checkpoint or on acute down-regulation of DNA replication after a radiation challenge.

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页码：177 / 183

页数：7

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