Wnt activates the Tak1/Nemo-like kinase pathway

被引:95
作者
Smit, L [1 ]
Baas, A [1 ]
Kuipers, J [1 ]
Korswagen, H [1 ]
van de Wetering, M [1 ]
Clevers, H [1 ]
机构
[1] Ctr Biomed Genet, Hubrecht Lab, NL-3584 CT Utrecht, Netherlands
关键词
D O I
10.1074/jbc.M307801200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Genetic studies on endoderm-mesoderm specification in Caenorhabditis elegans have demonstrated a role for several Wnt cascade components as well as for a MAPK-like pathway in this process. The latter pathway includes the MAPK kinase kinase-like MOM-4/Tak1, its adaptor TAP-1/Tab1, and the MAPK-like LIT-1/Nemolike kinase. A model has been proposed in which the Tak1 kinase cascade counteracts the Wnt cascade at the level of beta-catenin/TCF phosphorylation. In this model, the signal that activates the Tak1 kinase cascade is unknown. As an alternative explanation of these genetic data, we have explored whether Tak1 is directly activated by Wnt. We find that Wnt1 stimulation results in autophosphorylation and activation of MOM-4/Tak1 in a TAP-1/Tab1-dependent fashion. Wnt1-induced Tak1 stimulation activates Nemo-like kinase, resulting in the phosphorylation of TCF. Our results combined with the genetic data from C. elegans imply a mechanism whereby Wnt directly activates the MOM-4/Tak1 kinase signaling pathway. Thus, Wnt signal transduction through the canonical pathway activates beta-catenin/TCF, whereas Wnt signal transduction through the Tak1 pathway phosphorylates and inhibits TCF, which might function as a feedback mechanism.
引用
收藏
页码:17232 / 17240
页数:9
相关论文
共 49 条
[41]   Wnt signaling polarizes an early C-elegans blastomere to distinguish endoderm from mesoderm [J].
Thorpe, CJ ;
Schlesinger, A ;
Carter, JC ;
Bowerman, B .
CELL, 1997, 90 (04) :695-705
[42]   Wnt-5a inhibits the canonical Wnt pathway by promoting GSK-3-independent β-catenin degradation [J].
Topol, L ;
Jiang, XY ;
Choi, H ;
Garrett-Beal, L ;
Carolan, PJ ;
Yang, YZ .
JOURNAL OF CELL BIOLOGY, 2003, 162 (05) :899-908
[43]   The β-catenin/TCF-4 complex imposes a crypt progenitor phenotype on colorectal cancer cells [J].
van de Wetering, M ;
Sancho, E ;
Verweij, C ;
de Lau, W ;
Oving, I ;
Hurlstone, A ;
van der Horn, K ;
Batlle, E ;
Coudreuse, D ;
Haramis, AP ;
Tion-Pon-Fong, M ;
Moerer, P ;
van den Born, M ;
Soete, G ;
Pals, S ;
Eilers, M ;
Medema, R ;
Clevers, H .
CELL, 2002, 111 (02) :241-250
[44]   TAK1 is a ubiquitin-dependent kinase of MKK and IKK [J].
Wang, C ;
Deng, L ;
Hong, M ;
Akkaraju, GR ;
Inoue, J ;
Chen, ZJJ .
NATURE, 2001, 412 (6844) :346-351
[45]   Activation of the hematopoietic progenitor kinase-1 (HPK1)-dependent, stress-activated c-Jun N-terminal kinase (JNK) pathway by transforming growth factor beta (TGF-beta)-activated kinase (TAK1), a kinase mediator of TGF beta signal transduction [J].
Wang, WF ;
Zhou, GS ;
Hu, MCT ;
Yao, ZB ;
Tan, TH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (36) :22771-22775
[46]   Mechanisms of Wnt signaling in development [J].
Wodarz, A ;
Nusse, R .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1998, 14 :59-88
[47]   Ephrin-B1 reverse signaling activates JNK through a novel mechanism that is independent of tyrosine phosphorylation [J].
Xu, Z ;
Lai, KO ;
Zhou, HM ;
Lin, SC ;
Ip, NY .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (27) :24767-24775
[48]   IDENTIFICATION OF A MEMBER OF THE MAPKKK FAMILY AS A POTENTIAL MEDIATOR OF TGF-BETA SIGNAL-TRANSDUCTION [J].
YAMAGUCHI, K ;
SHIRAKABE, T ;
SHIBUYA, H ;
IRIE, K ;
OISHI, I ;
UENO, N ;
TANIGUCHI, T ;
NISHIDA, E ;
MATSUMOTO, K .
SCIENCE, 1995, 270 (5244) :2008-2011
[49]   Naked cuticle encodes an inducible antagonist of Wnt signalling [J].
Zeng, WL ;
Wharton, KA ;
Mack, JA ;
Wang, K ;
Gadbaw, M ;
Suyama, K ;
Klein, PS ;
Scott, MP .
NATURE, 2000, 403 (6771) :789-795