Cell response of nanographene platelets to human osteoblast-like MG63 cells

被引：23

作者：

Zhang, X. ^{[1
]}

Li, M. ^{[1
]}

Wang, Y. B. ^{[1
,2
]}

Cheng, Y. ^{[1
]}

Zheng, Y. F. ^{[1
,3
]}

Xi, T. F. ^{[1
]}

Wei, S. C. ^{[1
,4
]}

机构：

[1] Peking Univ, Ctr Biomed Mat & Tissue Engn, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China

[2] Angstron Mat LLC, Dayton, OH 45404 USA

[3] Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China

[4] Peking Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2014年 / 102卷 / 03期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

nanographene platelets; cytotoxicity; MG63; cells; LDH; ALP; GRAPHENE OXIDE; CARBON NANOTUBES; NANOCOMPOSITES; DELIVERY;

D O I：

10.1002/jbm.a.34751

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

The biologic/cytotoxic effects of dispersed nanographene platelets (NGPs) on human osteosarcoma cells (MG63 cell line) were first studied by examining cell viability, cycle, apoptosis, change in morphology, lactate dehydrogenase (LDH) release, alkaline phosphatase (ALP) activity, and inflammation. The results shown that the cell cytotoxicity of the dispersed NGPs exhibited dose-dependent characters, which had no obvious cytotoxic effects to MG63 cells at the concentration less than 10g mL(-1), whereas could postpone cell cycle, promote cell apoptosis, damage cell microstructure, induce serious tumor necrosis factor- expression and greatly reduce ALP activity of MG63 cells at higher concentration of NGPs (>10 mu g mL(-1)). Besides, NGPs had little influence on the LDH leakage. The cytotoxic mechanism of NGPs to MG63 cells was speculated to be intracellular activity with no physical damage of plasma membrane. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 732-742, 2014.

引用

页码：732 / 742

页数：11

共 42 条

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