Synthesis and hybridization properties of DNA-PNA chimeras carrying 5-bromouracil and 5-methylcytosine

被引：24

作者：

Ferrer, E ^{[1
]}

Shevchenko, A ^{[1
]}

Eritja, R ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2000年 / 8卷 / 02期

关键词：

5-bromouracil; duplex stability; 5-methylcytosine; PNA; PNA-DNA chimeras;

D O I：

10.1016/S0968-0896(99)00308-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation of 5-bromouracil and 5-methylcytosine peptide nucleic acid (PNA) monomers is described. These PNA monomers were used for the preparation of several DNA-PNA chimeras and their hybridization properties are described. The substitution of cytosine by 5-methylcytosine in DNA-PNA chimeras increased duplex stability while substitution of thymine by 5-bromouracil maintained it. Moreover, binding of DNA-PNA chimeras to double-stranded DNA to form triple helices was studied. In contrast to DNA, the presence of 5-methylcytosine and 5-bromouracil in DNA-PNA chimeras destabilized triple helix. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：291 / 297

页数：7

共 27 条

[1] SOLID-PHASE SYNTHESIS OF DIRECTLY LINKED PNA-DNA-HYBRIDS [J].