Characterization of protein immobilization on alkyl monolayer modified silicon(111) surface

被引：41

作者：

Liao, W ^{[1
]}

Wei, F ^{[1
]}

Qian, MX ^{[1
]}

Zhao, XS ^{[1
]}

机构：

[1] Peking Univ, Coll Chem, Dept Biol Chem, State Key Lab Mol Dynam & Stable Struct, Beijing 100871, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2004年 / 101卷 / 03期

基金：

中国国家自然科学基金;

关键词：

alkyl monolayer; silicon(111); immunoglobulin; surface protein density; affinity; homogeneity;

D O I：

10.1016/j.snb.2004.04.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Protein chip, a powerful high-throughput tool to study protein function, plays a prominent role in the basic research and drug discovery. We have applied a systematic procedure to characterize and evaluate the surface chemistry on the well-known alkyl monolayer modified Si(111) surface. Protein molecules are immobilized on the surface through chemical bonds, and the specific recognition is observed. The surface protein density has been determined by the combination of Atomic Force Microscopy and immobilization kinetics. The affinity of the surface immobilized IgG molecules is examined by fluorescence detection incorporated with the Langmuir isotherm. In addition, specificity, sensitivity, and homogeneity are also discussed. (C) 2004 Elsevier B.V All rights reserved.

引用

页码：361 / 367

页数：7

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