Microfluidic devices for measuring gene network dynamics in single cells

被引：188

作者：

Bennett, Matthew R. ^{[1
,2
]}

Hasty, Jeff ^{[3
,4
]}

机构：

[1] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA

[2] Rice Univ, Inst Biosci & Bioengn, Houston, TX 77005 USA

[3] Univ Calif San Diego, Dept Mol Biol, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Dept Bioengn, BioCircuits Inst, La Jolla, CA 92093 USA

来源：

NATURE REVIEWS GENETICS | 2009年 / 10卷 / 09期

基金：

美国国家卫生研究院;

关键词：

ON-A-CHIP; SACCHAROMYCES-CEREVISIAE; FLUORESCENT PROTEINS; SYSTEMS BIOLOGY; YEAST-CELLS; QUANTITATIVE-ANALYSIS; BACTERIAL CHEMOTAXIS; MESSENGER-RNA; EXPRESSION; GRADIENTS;

D O I：

10.1038/nrg2625

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The dynamics governing gene regulation have an important role in determining the phenotype of a cell or organism. From processing extracellular signals to generating internal rhythms, gene networks are central to many time-dependent cellular processes. Recent technological advances now make it possible to track the dynamics of gene networks in single cells under various environmental conditions using microfluidic 'lab-on-a-chip' devices, and researchers are using these new techniques to analyse cellular dynamics and discover regulatory mechanisms. These technologies are expected to yield novel insights and allow the construction of mathematical models that more accurately describe the complex dynamics of gene regulation.

引用

页码：628 / 638

页数：11

共 130 条

[31] A microfabricated fluorescence-activated cell sorter
Fu, AY
Spence, C
Scherer, A
Arnold, FH
Quake, SR
[J]. NATURE BIOTECHNOLOGY, 1999, 17 (11) : 1109 - 1111
[32] An integrated microfabricated cell sorter
Fu, AY
Chou, HP
Spence, C
Arnold, FH
Quake, SR
[J]. ANALYTICAL CHEMISTRY, 2002, 74 (11) : 2451 - 2457
[33] Construction of a genetic toggle switch in Escherichia coli
Gardner, TS
Cantor, CR
Collins, JJ
[J]. NATURE, 2000, 403 (6767) : 339 - 342
[34] Single-cell protein induction dynamics reveals a period of vulnerability to antibiotics in persister bacteria
Gefen, Orit
Gabay, Chana
Mumcuoglu, Michael
Engel, Giora
Balaban, Nathalie Q.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (16) : 6145 - 6149
[35] EXACT STOCHASTIC SIMULATION OF COUPLED CHEMICAL-REACTIONS
GILLESPIE, DT
[J]. JOURNAL OF PHYSICAL CHEMISTRY, 1977, 81 (25) : 2340 - 2361
[36] LOGICAL ANALYSIS OF CONTINUOUS, NONLINEAR BIOCHEMICAL CONTROL NETWORKS
GLASS, L
KAUFFMAN, SA
[J]. JOURNAL OF THEORETICAL BIOLOGY, 1973, 39 (01) : 103 - 129
[37] Real-time kinetics of gene activity in individual bacteria
Golding, I
Paulsson, J
Zawilski, SM
Cox, EC
[J]. CELL, 2005, 123 (06) : 1025 - 1036
[38] A synthetic gene network for tuning protein degradation in Saccharomyces cerevisiae
Grilly, Chris
Stricker, Jesse
Pang, Wyming Lee
Bennett, Matthew R.
Hasty, Jeff
[J]. MOLECULAR SYSTEMS BIOLOGY, 2007, 3 (1)
[39] A microfluidic chemostat for experiments with bacterial and yeast cells
Groisman, A
Lobo, C
Cho, HJ
Campbell, JK
Dufour, YS
Stevens, AM
Levchenko, A
[J]. NATURE METHODS, 2005, 2 (09) : 685 - 689
[40] A genomic integration method to visualize localization of endogenous mRNAs in living yeast
Haim, Liora
Zipor, Gadi
Aronov, Stella
Gerst, Jeffrey E.
[J]. NATURE METHODS, 2007, 4 (05) : 409 - 412

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