A Facile Method for Preparation of Tailored Scaffolds for DNA-Origami

被引：39

作者：

Erkelenz, Michael ^{[1
]}

Bauer, Dennis M. ^{[1
,2
]}

Meyer, Rebecca ^{[1
,5
]}

Gatsogiannis, Christos ^{[3
]}

Raunser, Stefan ^{[3
]}

Sacca, Barbara ^{[1
,4
]}

Niemeyer, Christof M. ^{[1
,5
]}

机构：

[1] Tech Univ Dortmund, Fak Chem, D-44227 Dortmund, Germany

[2] Karlsruhe Inst Technol KIT, DFG Ctr Funct Nanostruct CFN, D-76131 Karlsruhe, Germany

[3] Max Planck Inst Mol Physiol, D-44227 Dortmund, Germany

[4] Univ Duisburg Essen, Zentrum Med Biotechnol ZMB, Fak Biol, D-45117 Essen, Germany

[5] Karlsruhe Inst Technol KIT, Inst Biol Interfaces IBG 1, D-76344 Eggenstein Leopoldshafen, Germany

来源：

SMALL | 2014年 / 10卷 / 01期

关键词：

bacterial cloning; DNA; nanostructures; self-assembly; FOLDING DNA; NANOSCALE SHAPES;

D O I：

10.1002/smll.201300701

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

A convenient PCR cloning strategy allows one to prepare hundreds of picomoles of circular single-stranded DNA molecules, which are suitable as scaffolds for the assembly of DNA origami structures. The method is based on a combination of site-directed mutagenesis and site- and ligation-independent cloning protocols, with simultaneous insertion of a nicking endonuclease restriction site on a double-stranded plasmid of desired length and sequence. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：73 / 77

页数：5

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