Shear and AC Field Enhanced Carbon Nanotube Impedance Assay for Rapid, Sensitive, and Mismatch-Discriminating DNA Hybridization

被引:73
作者
Basuray, Sagnik [1 ]
Senapati, Satyajyoti [1 ]
Aijian, Andrew [1 ]
Mahon, Andrew R. [2 ]
Chang, Hsueh-Chia [1 ]
机构
[1] Univ Notre Dame, Dept Chem & Biomol Engn, Notre Dame, IN 46556 USA
[2] Univ Notre Dame, Dept Biol Sci, Ctr Aquat Conservat, Notre Dame, IN 46556 USA
关键词
bioassay; carbon nanotube; DNA hybridization; impedance; open flow; BIOSENSOR;
D O I
10.1021/nn9004632
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Other than concentrating the target molecules at the sensor location, we demonstrate two distinct new advantages of an open-flow impedance-sensing platform for DNA hybridization on carbon nanotube (CNT) surface in the presence of a high-frequency AC electric field. The shear-enhanced DNA and ion transport rate to the CNT surface decouples the parasitic double-layer AC impedance signal from the charge-transfer signal due to DNA hybridization. The flow field at high AC frequency also amplifies the charge-transfer rate across the hybridized CNT and provides shear-enhanced discrimination between DNA from targeted species and a closely related congeneric species with three nucleotide mismatches out of 26 bases in a targeted attachment region. This allows sensitive detection of hybridization events in less than 20 min with picomolar target DNA concentrations in a label-free CNT-based microfluidic detection platform.
引用
收藏
页码:1823 / 1830
页数:8
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