Confirmatory and quantitative analysis using experimental design for the extraction and liquid chromatography-UV, liquid chromatography-mass spectrometry and liquid chromatography-mass spectrometry/mass spectrometry determination of quinolones in turkey muscle

被引：50

作者：

Clemente, M. ^{[1
]}

Hermo, M. P. ^{[1
]}

Barron, D. ^{[1
]}

Barbosa, J. ^{[1
]}

机构：

[1] Univ Barcelona, Dept Analyt Chem, E-08028 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1135卷 / 02期

关键词：

liquid chromatography; mass spectrometry; tandem mass spectrometry; quinolones; experimental design; stability studies;

D O I：

10.1016/j.chroma.2006.09.041

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this work is to established methods for determination of quinolones (ciprofloxacin, danofloxacin, enrofloxacin, difloxacin and flumequine), regulated by European Union, and sarafloxacin in turkey muscle. An experimental design has been applied for the optimization of the factors that influence the extraction of quinolones from turkey muscle in order to determine the experimental conditions for their extraction with high recoveries. Liquid chromatography with ultraviolet detection (LC-UV), liquid chromatography-mass spectrometry (LC-MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS) have been used for the simultaneous quantification of quinolones antibiotics in turkey muscle. The proposed methods have been validated according to the Food Drugs Administration guideline and presents the limit of quantification below the maximum residue limits established by the European Union for quinolones in turkey muscle. The methods developed have been applied to quantification of enrofloxacin and its main metabolite ciprolloxacin in samples of turkey muscle obtained from animals treated with enrofloxacin. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：170 / 178

页数：9

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