A new kinetic model proposed for enzymatic hydrolysis of lactose by a β-galactosidase from Kluyveromyces fragilis

被引：78

作者：

Jurado, E ^{[1
]}

Camacho, F ^{[1
]}

Luzón, G ^{[1
]}

Vicaria, JM ^{[1
]}

机构：

[1] Univ Granada, Dept Ingn Quim, Fac Ciencias, E-18071 Granada, Spain

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2002年 / 31卷 / 03期

关键词：

lactose hydrolysis; Kluyveromyces fragilis; kinetic model; beta-galactosidase;

D O I：

10.1016/S0141-0229(02)00107-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We study the enzymatic hydrolysis of lactose by a commercial enzyme from a selected strain of Kluyveromyces fragilis. The variables analyzed were: temperature (25-40degreesC), enzyme concentration (0.1-3.0 g l(-1)), lactose concentration (0.0278-0.208 M), and initial galactose concentration (0.0347 M). On the basis of the data analyzed, both published and in the present work, we propose a Michaelis-Menten kinetic model with inhibition by the product (galactose), which reveals that the substrate (lactose) and the product (galactose) present similar affinity for the active site of the enzyme. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：300 / 309

页数：10

共 38 条

[31]

SHAH NP, 1993, MILCHWISSENSCHAFT, V48, P494

[32] Continuous production of galacto-oligosaccharides from lactose by Bullera singularis β-galactosidase immobilized in chitosan beads [J].

Shin, HJ ;

Park, JM ;

Yang, JW .

PROCESS BIOCHEMISTRY, 1998, 33 (08) :787-792

[33] Batch and continuous hydrolysis of lactose using β-galactosidase immobilized on gelatin-CMC [J].

Sungur, S ;

Yildirim, Ö .

POLYMER-PLASTICS TECHNOLOGY AND ENGINEERING, 1999, 38 (04) :821-829

[34]

WALSH MK, 1993, J FOOD BIOCHEM, V17, P283

[35]

WERNER W, 1970, FRESEN Z ANAL CHEM, V252, P224

[36] EFFECTS OF TEMPERATURE ON LACTOSE HYDROLYSIS BY IMMOBILIZED BETA-GALACTOSIDASE IN PLUG-FLOW REACTOR [J].

YANG, ST ;

OKOS, MR .

BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (07) :873-885

[37] A NEW GRAPHICAL-METHOD FOR DETERMINING PARAMETERS IN MICHAELIS MENTEN-TYPE KINETICS FOR ENZYMATIC LACTOSE HYDROLYSIS [J].

YANG, ST ;

OKOS, MR .

BIOTECHNOLOGY AND BIOENGINEERING, 1989, 34 (06) :763-773

[38]

Zadow J. G., 1992, Whey and lactose processing., P361

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