A soft and flexible biosensor using a phospholipid polymer for continuous glucose monitoring

被引：27

作者：

Chu, MingXing ^{[2
]}

Kudo, Hiroyuki ^{[1
]}

Shirai, Takayuki ^{[3
]}

Miyajima, Kumiko ^{[1
]}

Saito, Hirokazu ^{[1
,4
]}

Morimoto, Nobuyuki ^{[5
,6
]}

Yano, Kazuyoshi ^{[7
]}

Iwasaki, Yasuhiko ^{[8
]}

Akiyoshi, Kazunari ^{[5
]}

Mitsubayashi, Kohji ^{[1
]}

机构：

[1] Tokyo Med & Dent Univ, Dept Biomed Devices & Instrumentat, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan

[2] Tokyo Med & Dent Univ, Grad Sch Med & Dent Sci, Chiyoda Ku, Tokyo 1010062, Japan

[3] Tokyo Univ Technol, Grad Sch, Tokyo 1920982, Japan

[4] Tokyo Natl Coll Technol, Dept Mech Engn, Tokyo 1930997, Japan

[5] Tokyo Med & Dent Univ, Dept Organ Mat, Inst Biomat & Bioengn, Chiyoda Ku, Tokyo 1010062, Japan

[6] Tohoku Univ, Dept Mat Proc, Grad Sch Engn, Sendai, Miyagi 9808579, Japan

[7] Tokyo Univ Technol, Sch Bion, Tokyo 1920982, Japan

[8] Kansai Univ, Dept Chem & Mat Engn, Fac Chem Mat & Bioengn, Suitasi, Osakafu 5648680, Japan

来源：

BIOMEDICAL MICRODEVICES | 2009年 / 11卷 / 04期

基金：

日本学术振兴会;

关键词：

Biosensor; Phospholipid polymer; Flexible; Glucose sensor; TEAR GLUCOSE; SENSOR; TISSUE; ELECTRODES;

D O I：

10.1007/s10544-009-9300-1

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A flexible biosensor using a phospholipid polymer to immobilization of glucose oxidase (GOD) was fabricated and tested. At first, an enzyme membrane formed by immobilizing GOD onto a porous polytetrafluoroethylene (PTFE) membrane using the phospholipid polymer (2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with 2-ethylhexylmethacrylate (EHMA) : PMEH) was evaluated. According to the result of amperometric measurement, average density of GOD to be immobilized was optimized to 38.9 units cm(-2). Temperature and pH dependences were also investigated. Then, a flexible glucose sensor was fabricated by immobilizing GOD onto a flexible hydrogen peroxide electrode using PMEH. The flexible glucose sensor showed a linear relationship between output currents and glucose concentration in 0.05-1.00 mmol L-1, with a correlation coefficient of 0.999. The calibration range covered the normal tear glucose level of 0.14-0.23 mmol L-1. This indicates that the flexible biosensor is considered to be useful for monitoring of glucose in tear fluids.

引用

页码：837 / 842

页数：6

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