Surfactant protein D reduces alveolar macrophage apoptosis in vivo

被引:126
作者
Clark, H
Palaniyar, N
Strong, P
Edmondson, J
Hawgood, S
Reid, KBM
机构
[1] Univ Oxford, MRC, Immunochem Unit, Dept Biochem, Oxford OX1 3QU, England
[2] Univ Calif San Francisco, Dept Pediat, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Inst Cardiovasc Res, San Francisco, CA 94143 USA
关键词
D O I
10.4049/jimmunol.169.6.2892
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Surfactant protein D (SP-D) is a molecule of the innate immune system that recognizes the patterns of surface carbohydrate on pathogens and targets them for phagocytosis and killing. SP-D-deficient mice show an increased number of macrophages in the alveolar space, excess surfactant phospholipid, overproduction of reactive oxygen species, and the development of emphysema. We report here that SP-D-deficient mice have a 5- to 10-fold increase in the number of apoptotic and necrotic alveolar macrophages, as defined by annexin V and propidium iodine staining, respectively. Intrapulmonary administration of a truncated 60-kDa fragment of human recombinant SP-D reduces the number of apoptotic and necrotic alveolar macrophages and partially corrects the lipid accumulation in SP-D-deficient mice. The same SP-D fragment binds preferentially to apoptotic and necrotic alveolar macrophages in vitro, suggesting that SP-D contributes to immune homeostasis in the lung by recognizing and promoting removal of necrotic and apoptotic cells.
引用
收藏
页码:2892 / 2899
页数:8
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