The effect of ultra-nanocrystalline diamond films on the proliferation and differentiation of neural stem cells

被引：62

作者：

Chen, Ying-Chieh ^{[1
,2
]}

Lee, Don-Ching ^{[2
]}

Hsiao, Chao-Yang ^{[2
]}

Chung, Yu-Fen ^{[2
]}

Chen, Huang-Chin ^{[3
]}

Thomas, Joseph P. ^{[1
]}

Pong, Way-Faung ^{[3
]}

Tai, Nyan-Hwa ^{[1
]}

Lin, I-Nan ^{[3
]}

Chiu, Ing-Ming ^{[2
,4
]}

机构：

[1] Natl Tsing Hua Univ, Dept Mat Sci & Engn, Hsinchu 300, Taiwan

[2] Natl Hlth Res Inst, Inst Cellular & Syst Med, Jhunan 35053, Miaoli, Taiwan

[3] Tamkang Univ, Dept Phys, Tamsui 251, Taiwan

[4] Ohio State Univ, Dept Internal Med, Columbus, OH 43210 USA

来源：

BIOMATERIALS | 2009年 / 30卷 / 20期

关键词：

Neural stem cells; Neural differentiation; Ultra-nanocrystalline diamond; ECM; ULTRANANOCRYSTALLINE DIAMOND; STEM/PROGENITOR CELLS; GROWTH-FACTOR; THIN-FILMS; IN-VITRO; BIOCOMPATIBILITY; SURFACES; SILICON; BMP-2;

D O I：

10.1016/j.biomaterials.2009.03.058

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The interaction of ultra-nanocrystalline diamond (UNCD) with neural stem cells (NSCs) has been studied along with its surface modification in order to improve its function as a biomaterial. Hydrogen- and oxygen-terminated UNCD films were compared with standard grade polystyrene in terms of their impact on the growth, expansion and differentiation of NSCs. When NSCs were cultured on these substrates in low serum and without any differentiating factors, hydrogen-terminated UNCD films spontaneously induced cell proliferation and neuronal differentiation. Oxygen-terminated UNCD films were also shown to further improve neural differentiation, with a preference to differentiate into oligodendrocytes. Hence, controlling the surface properties of UNCD could manipulate the differentiation of NSCs for different biomedical applications. These observations raise the potential for the use of UNCD as a biomaterial for central nervous system transplantation and tissue engineering. (c) 2009 Elsevier Ltd. All rights reserved.

引用

页码：3428 / 3435

页数：8

共 48 条

[1] Nanocrystalline diamond:: In vitro biocompatibility assessment by MG63 and human bone marrow cells cultures [J].