Multitrack electrospray chips

被引：26

作者：

Dayon, Loic ^{[1
]}

Abonnenc, Melanie ^{[1
]}

Prudent, Michel ^{[1
]}

Lion, Niels ^{[1
]}

Girault, Hubert H. ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Electrochem Phys & Analyt, Stn 6, CH-1015 Lausanne, Switzerland

来源：

JOURNAL OF MASS SPECTROMETRY | 2006年 / 41卷 / 11期

关键词：

chip; electrospray; emitter; mass spectrometry; quantification; sequential; source; tagging;

D O I：

10.1002/jms.1119

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Multitrack electrospray chips (MTEC) were fabricated by UV-photoablation of polyethylene terephthalate (PET) substrates. They are composed of an array of up to six microchannels that are successively used as electrospray ionization (ESI) emitters for mass spectrometry (MS). There is no requirement for alignment of the different spraying microchannels with the mass spectrometer orifice. The MTEC is thus fixed in front of the mass spectrometer and the successive MS analyses are performed without moving the chip. The sequential electrospraying by successive application of an identical high voltage in each off-axis microchannel was evaluated for the fast screening of peptides and proteins. The counting of cysteines in peptides through chemical modification and the relative quantification of a peptide in two samples are presented herein as two original strategies based on this new analytical tool. Copyright (c) 2006 John Wiley & Sons, Ltd.

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页码：1484 / 1490

页数：7

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