The puromycin route to assess stereo- and regiochemical constraints on peptide bond formation in eukaryotic ribosomes

被引：48

作者：

Starck, SR ^{[1
]}

Qi, X ^{[1
]}

Olsen, BN ^{[1
]}

Roberts, RW ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 27期

关键词：

D O I：

10.1021/ja034817e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We synthesized a series of puromycin analogues to probe the chemical specificity of the ribosome in an intact eukaryotic translation system. These studies reveal that both d-enantiomers and β-amino acid analogues can be incorporated into protein, and provide a quantitative means to rank natural and unnatural residues. Modeling of a d-amino acid analogue into the 50S ribosomal subunit indicates that steric clash may provide part of the chiral discrimination. The data presented provide one metric of the chiral and regiospecificity of mammalian ribosomes. Copyright © 2003 American Chemical Society.

引用

收藏

页码：8090 / 8091

页数：2

相关论文

共 34 条

[1] The tRNA specificity of Thermus thermophilus EF-Tu [J].

Asahara, H ;

Uhlenbeck, OC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (06) :3499-3504

[2] SITE-SPECIFIC INCORPORATION OF NONNATURAL RESIDUES INTO PEPTIDES - EFFECT OF RESIDUE STRUCTURE ON SUPPRESSION AND TRANSLATION EFFICIENCIES [J].

BAIN, JD ;

WACKER, DA ;

KUO, EE ;

CHAMBERLIN, AR .

TETRAHEDRON, 1991, 47 (14-15) :2389-2400

[3] BIOSYNTHETIC SITE-SPECIFIC INCORPORATION OF A NON-NATURAL AMINO-ACID INTO A POLYPEPTIDE [J].

BAIN, JD ;

GLABE, CG ;

DIX, TA ;

CHAMBERLIN, AR ;

DIALA, ES .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (20) :8013-8014

[4] AMINOACYL DERIVATIVES OF NUCLEOSIDES, NUCLEOTIDES AND POLYNUCLEOTIDES .36. THE PEPTIDYLTRANSFERASE CENTER OF ESCHERICHIA-COLI RIBOSOMES - BINDING-SITES FOR THE CYTIDINE 3'-PHOSPHATE RESIDUES OF THE AMINOACYL-TRNA 3'-TERMINUS AND THE INTERRELATIONSHIPS BETWEEN THE ACCEPTOR AND DONOR SITES [J].

BHUTA, P ;

KUMAR, G ;

CHLADEK, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 696 (02) :208-211

[5] HYDROLYSIS OF GTP ON ELONGATION-FACTOR TU.RIBOSOME COMPLEXES PROMOTED BY 2'(3')-O-L-PHENYLALANYLADENOSINE [J].

CAMPUZANO, S ;

MODOLELL, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (02) :905-909

[6] THE 3'-END OF TRANSFER-RNA AND ITS ROLE IN PROTEIN-BIOSYNTHESIS [J].

CHLADEK, S ;

SPRINZL, M .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1985, 24 (05) :371-391

[7] PROBING PROTEIN-STRUCTURE AND FUNCTION WITH AN EXPANDED GENETIC-CODE [J].

CORNISH, VW ;

MENDEL, D ;

SCHULTZ, PG .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1995, 34 (06) :621-633

[8] Site-specific incorporation of (aminooxy)acetic acid into proteins [J].

Eisenhauer, BM ;

Hecht, SM .

BIOCHEMISTRY, 2002, 41 (38) :11472-11478

[9]

ELLMAN J, 1991, METHOD ENZYMOL, V202, P301

[10] Backbone mutations in transmembrane domains of a ligand-gated ion channel: Implications for the mechanism of gating [J].

England, PM ;

Zhang, YN ;

Dougherty, DA ;

Lester, HA .

CELL, 1999, 96 (01) :89-98

← 1 2 3 4 →