Synthesis of immunocomponents for the measurement of lead (Pb) by fluorescence polarization immunoassay

被引：19

作者：

Adamczyk, M ^{[1
]}

Fishpaugh, JR ^{[1
]}

Heuser, KJ ^{[1
]}

Ramp, JM ^{[1
]}

Reddy, RE ^{[1
]}

Wong, M ^{[1
]}

机构：

[1] Abbott Labs, Div Organ Chem Res 9NM AP20, Div Diagnost, Abbott Pk, IL 60064 USA

来源：

TETRAHEDRON | 1998年 / 54卷 / 13期

关键词：

D O I：

10.1016/S0040-4020(98)00060-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Three novel haptens (5b, 9b, 11b) were synthesized from porphobilinogen (2) and conjugated to bovine serum albumin to afford immunogens (6, 10, 12). Five fluorescent tracers (14, 17, 19, 29, 32) were prepared from porphobilinogen (2), or haptens (9b, 11b, 20b) in >98% purity. The immunogens (6, 10, 12) were utilized for generation of polyclonal antibodies and the best tracer-antibody combination was selected to construct the calibration curve for the measurement of lead. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：3093 / 3112

页数：20

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