Standing wave total internal reflection fluorescence microscopy to measure the size of nanostructures in living cells

被引：23

作者：

Gliko, Olga

Reddy, Gaddum D.

Anvari, Bahman

Brownell, William E.

Saggau, Peter

机构：

[1] Baylor Coll Med, Dept Neurosci, Houston, TX 77030 USA

[2] Baylor Coll Med, Dept Otolaryngol Head & Neck Surg, Houston, TX 77030 USA

[3] Rice Univ, Dept Bioengn, Houston, TX 77005 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2006年 / 11卷 / 06期

关键词：

resolution; fluorescence; interference; phase shifts; image reconstruction; point spread functions;

D O I：

10.1117/1.2372457

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present the first application of standing wave fluorescence microscopy (SWFM) to determine the size of biological nanostructures in living cells. The improved lateral resolution of less than 100 nm enables superior quantification of the size of subcellular structures. We demonstrate the ability of SWFM by measuring the diameter of biological nanotubes (membrane tethers formed between cells). The combination of SWFM with total internal reflection (TIR), referred to as SW-TIRFM, allows additional improvement of axial resolution by selective excitation of fluorescence in a layer of about 100 nm. (c) 2006 Society of Photo-Optical Instrumentation Engineers.

引用

页数：5

共 14 条

[1] CELL-SUBSTRATE CONTACTS ILLUMINATED BY TOTAL INTERNAL-REFLECTION FLUORESCENCE [J].

AXELROD, D .

JOURNAL OF CELL BIOLOGY, 1981, 89 (01) :141-145

[2] Extended resolution wide-field optical imaging: objective-launched standing-wave total internal reflection fluorescence microscopy [J].

Chung, E ;

Kim, DK ;

So, PTC .

OPTICS LETTERS, 2006, 31 (07) :945-947

[3] Lateral resolution enhancement with standing evanescent waves [J].

Cragg, GE ;

So, PTC .

OPTICS LETTERS, 2000, 25 (01) :46-48

[4] MECHANICAL-PROPERTIES OF NEURONAL GROWTH CONE MEMBRANES STUDIED BY TETHER FORMATION WITH LASER OPTICAL TWEEZERS [J].

DAI, JW ;

SHEETZ, MP .

BIOPHYSICAL JOURNAL, 1995, 68 (03) :988-996

[5] Fast 100-nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast [J].

Egner, A ;

Jakobs, S ;

Hell, SW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (06) :3370-3375

[6] Nanosizing of fluorescent objects by spatially modulated illumination microscopy [J].

Failla, AV ;

Spoeri, U ;

Albrecht, B ;

Kroll, A ;

Cremer, C .

APPLIED OPTICS, 2002, 41 (34) :7275-7283

[7] True optical resolution beyond the Rayleigh limit achieved by standing wave illumination [J].

Frohn, JT ;

Knapp, HF ;

Stemmer, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (13) :7232-7236

[8] Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation [J].

Frohn, JT ;

Knapp, HF ;

Stemmer, A .

OPTICS LETTERS, 2001, 26 (11) :828-830

[9] Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution [J].

Gustafsson, MGL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (37) :13081-13086

[10] Image processing in 3-D standing-wave fluorescence microscopy [J].

Krishnamurthi, V ;

Bailey, B ;

Lanni, F .

THREE-DIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING III, 1996, 2655 :18-25

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