Chemiluminescence flow biosensor for hydrogen peroxide with immobilized reagents

被引：47

作者：

Li, BX ^{[1
]}

Zhang, ZJ ^{[1
]}

Jin, Y ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Dept Chem, Xian 710062, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2001年 / 72卷 / 02期

基金：

中国国家自然科学基金;

关键词：

chemiluminescence; flow biosensor; sol-gel method; hydrogen peroxide;

D O I：

10.1016/S0925-4005(00)00623-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel chemiluminescence (CL) flow biosensor for hydrogen peroxide is described. It is prepared by immobilizing HRP with the sol-gel method on the flow CL cell, and by electrostatically immobilizing luminol on a strongly basic anion-exchanger resin. Hydrogen peroxide is sensed by the CL reaction with HRP sensitive membrane and luminol bleeding from the ion-exchanger column with immobilized luminol by hydrolysis. The calibration graph is linear in the range of 1 x 10(-4)-8 x 10(-7) mol l(-1), and the detection limit is 2.8 x 10(-7) mol l(-1) hydrogen peroxide. A. complete analysis could be performed in I min with a relative standard deviation of 2.1% for 1 x 10(-6) mol l(-1) H2O2 (n = 11). The biosensor has been applied successfully to the determination of hydrogen peroxide in rainwater. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：115 / 119

页数：5

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