Chemiluminescence flow biosensor for hydrogen peroxide with immobilized reagents

A novel chemiluminescence (CL) flow biosensor for hydrogen peroxide is described. It is prepared by immobilizing HRP with the sol-gel method on the flow CL cell, and by electrostatically immobilizing luminol on a strongly basic anion-exchanger resin. Hydrogen peroxide is sensed by the CL reaction with HRP sensitive membrane and luminol bleeding from the ion-exchanger column with immobilized luminol by hydrolysis. The calibration graph is linear in the range of 1 x 10(-4)-8 x 10(-7) mol l(-1), and the detection limit is 2.8 x 10(-7) mol l(-1) hydrogen peroxide. A. complete analysis could be performed in I min with a relative standard deviation of 2.1% for 1 x 10(-6) mol l(-1) H2O2 (n = 11). The biosensor has been applied successfully to the determination of hydrogen peroxide in rainwater. (C) 2001 Elsevier Science B.V. All rights reserved.