Nanostructured zinc oxide platform for mycotoxin detection

被引：94

作者：

Ansari, Anees A. ^{[1
]}

Kaushik, Ajeet ^{[1
]}

Solanki, Pratima R. ^{[1
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

来源：

BIOELECTROCHEMISTRY | 2010年 / 77卷 / 02期

关键词：

Sol-gel; Zinc oxide; Impedimetric immunosensor; Mycotoxin; Ochratoxin-A; HORSERADISH-PEROXIDASE; IMMOBILIZATION; IMMUNOSENSOR; PROTEINS; MATRIX;

D O I：

10.1016/j.bioelechem.2009.06.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanostructured zinc oxide (Nano-ZnO) film has been deposited onto indium-tin-oxide (ITO) glass plate for co-immobilization of rabbit-immunoglubin antibodies (r-IgGs) and bovine serum albumin (BSA) for ochratoxin-A (OTA) detection. The results of X-ray diffraction (XRD) studies reveal the formation of Nano-ZnO with average particle size as similar to 5.0 nm. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) techniques have been used to characterize Nano-ZnO/ITO electrode and BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode. Electrochemical impedimetric response of BSA/r-IgGs/Nano-ZnO/ITO immunoelectrode obtained as a function of OTA concentration exhibits linearity as 0.006-0.01 nM/dm(3), detection limit of 0.006 nM/dm(3), response time as 25 s and sensitivity of 189 Omega/nM/dm(3) cm(-2) with a regression coefficient of 0.997. (c) 2009 Elsevier B.V. All rights reserved.

引用

页码：75 / 81

页数：7

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