Cloning and analysis of the replication origin and the telomeres of the large linear plasmid pSLA2-L in Streptomyces rochei

被引：36

作者：

Hiratsu, K ^{[1
]}

Mochizuki, S

Kinashi, H

机构：

[1] Hiroshima Univ, Dept Fermentat Technol, Higashihiroshima, Hiroshima 7398527, Japan

[2] Hiroshima Univ, Grad Sch Adv Sci Matter, Dept Mol Biotechnol, Higashihiroshima, Hiroshima 7398527, Japan

来源：

MOLECULAR AND GENERAL GENETICS | 2000年 / 263卷 / 06期

关键词：

Streptomyces; linear plasmid; replication origin; telomere;

D O I：

10.1007/PL00008689

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The replication origin and both terminal segments were cloned from the large linear plasmid pSLA2-L in Streptomyces rochei 7434AN4. The basic replicon consists of a 1.9-kb DNA fragment, which contains the genetic information required for autonomous replication in circular form. Sequence analysis revealed two ORFs, RepL1 and RepL2, with no similarity to any of the replication initiator proteins in the database. Deletion and mutational analysis showed that RepL1 is essential for replication and RepL2 has a subsidiary function. The origin of replication may be located 800 bp upstream of repL1. Sequencing of the left and right terminal segments revealed the presence of 12 palindromes. The sequence of the first 90 bp, including palindromes I-IV, shows great similarity to that of other Streptomyces linear chromosomes and plasmids. These results suggest that the internal replication origins of the linear replicons vary widely, in contrast to the high degree of conservation of their telomeres.

引用

页码：1015 / 1021

页数：7

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