Breeding of a Low Pyruvate-Producing Sake Yeast by Isolation of a Mutant Resistant to Ethyl α-Transcyanocinnamate, an Inhibitor of Mitochondrial Pyruvate Transport

被引：23

作者：

Horie, Kenta ^{[1
]}

Oba, Takahiro ^{[2
]}

Motomura, Saori ^{[1
]}

Isogai, Atsuko ^{[3
]}

Yoshimura, Takashi ^{[4
]}

Tsuge, Keisuke ^{[4
]}

Koganemaru, Kazuyoshi ^{[4
]}

Kobayashi, Genta ^{[1
]}

Kitagaki, Hiroshi ^{[1
]}

机构：

[1] Saga Univ, Fac Agr, Saga 8408502, Japan

[2] Fukuoka Ind Technol Ctr, Biotechnol & Food Res Inst, Fukuoka 8390861, Japan

[3] Natl Res Inst Brewing, Hiroshima 7390046, Japan

[4] Ind Technol Ctr Saga Prefecture, Saga 8490932, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2010年 / 74卷 / 04期

关键词：

sake; mitochondrial transport; yeast; pyruvate; breeding; SACCHAROMYCES-CEREVISIAE; (-)-CITRAMALIC ACID; BIOGENESIS; MUTATIONS; INDUCTION; STRAINS;

D O I：

10.1271/bbb.90373

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pyruvate is the key substance controlling the formation of diacetyl, acetaldehyde. and acetate during alcoholic fermentation. Here we report the breeding of a low pyruvate-producing sake yeast by isolation of a mutant resistant to ethyl alpha-transcyanocinnamate, an inhibitor of mitochondrial pyruvate transport. Mitochondrial function was involved in resistance to this substance and in the production of pyruvate by the mutants.

引用

页码：843 / 847

页数：5

共 31 条

[21] GENETIC SCREENING IN SACCHAROMYCES-CEREVISIAE FOR LARGE NUMBERS OF MITOCHONDRIAL POINT MUTATIONS WHICH AFFECT STRUCTURE AND FUNCTION OF CATALYTIC SUBUNITS OF CYTOCHROME-C-OXIDASE [J].