Simultaneous use of multiple fluorescent reporter dyes for glucose sensing in aqueous solution

被引：22

作者：

Cordes, David B. ^{[1
]}

Miller, Aaron ^{[1
]}

Gamsey, Soya ^{[1
]}

Singaram, Bakthan ^{[1
]}

机构：

[1] Univ Calif Santa Cruz, Dept Chem & Biochem, Santa Cruz, CA 95064 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 387卷 / 08期

关键词：

carbohydrates; glucose sensing; boronic acid; fluorescence; viologen;

D O I：

10.1007/s00216-007-1128-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The simultaneous use of several fluorescent reporter dyes in a multicomponent boronic acid-based glucose sensing system is reported. In one application, two dyes with widely different emission wavelengths are used to report changes in glucose concentration. A third glucose-insensitive dye was then added to act as a reference dye and provide for a ratiometric correction to the two reporter dye signals. The inclusion of such a reference dye reduces errors arising from sources such as fluctuations in lamp intensity and sample dilution.

引用

页码：2767 / 2773

页数：7

共 17 条

[1] Boronic acid substituted viologen based optical sugar sensors: modulated quenching with viologen as a method for monosaccharide detection [J].

Camara, JN ;

Suri, JT ;

Cappuccio, FE ;

Wessling, RA ;

Singaram, B .

TETRAHEDRON LETTERS, 2002, 43 (07) :1139-1141

[2] Fluorescent chemosensors for carbohydrates: A decade's worth of bright spies for saccharides in review [J].

Cao, HS ;

Heagy, MD .

JOURNAL OF FLUORESCENCE, 2004, 14 (05) :569-584

[3] A tautomeric zinc sensor for ratiometric fluorescence imaging: Application to nitric oxide-induced release of intracellular zinc [J].

Chang, CJ ;

Jaworski, J ;

Nolan, EM ;

Sheng, M ;

Lippard, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (05) :1129-1134

[4] The interaction of boronic acid-substituted viologens with pyranine: The effects of quencher charge on fluorescence quenching and glucose response [J].

Cordes, DB ;

Gamsey, S ;

Sharrett, Z ;

Miller, A ;

Thoniyot, P ;

Wessling, RA ;

Singaram, B .

LANGMUIR, 2005, 21 (14) :6540-6547

[5] Optical glucose detection across the visible spectrum using anionic fluorescent dyes and a viologen quencher in a two-component saccharide sensing system [J].

Cordes, DB ;

Miller, A ;

Gamsey, S ;

Sharrett, Z ;

Thoniyot, P ;

Wessling, R ;

Singaram, B .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2005, 3 (09) :1708-1713

[6] From observed to corrected luminescence intensity of solution systems: an easy-to-apply correction method for standard spectrofluorimeters [J].

Credi, A ;

Prodi, L .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 1998, 54 (01) :159-170

[7] Progress in boronic acid-based fluorescent glucose sensors [J].

Fang, H ;

Kaur, G ;

Wang, BH .

JOURNAL OF FLUORESCENCE, 2004, 14 (05) :481-489

[8] The effect of boronic acid-positioning in an optical glucose-sensing ensemble [J].

Gamsey, Soya ;

Baxter, Nichol A. ;

Sharrett, Zachary ;

Cordes, David B. ;

Olmstead, Marilyn M. ;

Wessling, Ritchie A. ;

Singaram, Bakthan .

TETRAHEDRON, 2006, 62 (26) :6321-6331

[9] Dual-labeled glucose binding protein for ratiometric measurements of glucose [J].

Ge, XD ;

Tolosa, L ;

Rao, G .

ANALYTICAL CHEMISTRY, 2004, 76 (05) :1403-1410

[10] Fluorescent saccharide receptors: A sweet solution to the design, assembly and evaluation of boronic acid derived PET sensors [J].

James, TD ;

Linnane, P ;

Shinkai, S .

CHEMICAL COMMUNICATIONS, 1996, (03) :281-288

← 1 2 →