Anti-inflammatory effect of Calycosin glycoside on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells

Objective to find the Calycosin glycoside (CG) potential anti-inflammatory effect. The anti-inflammatory activity of CG was evaluated by lipopolysaccharide (LPS)-induced RAW 264.7 cells, and the IL-6, IL-1 beta, TNF-alpha and PGE2 level were measured by ELISA; NO levels in the culture media were determined using the Griess reaction. The iNOS and COX-2 mRNA expressions were measured by qRT-PCR and the phosphorylation of I kappa B alpha, 65, ERK, JNK, and p38 was determined by western blotting. The CG could markedly inhibit the productions of NO and PGE(2) and markedly inhibit the productions of TNF-alpha, IL-1 beta and IL-6. CG could suppress mRNA expression of iNOS, COX-2 and protein phosphorylation of I kappa B alpha, p65, ERK, JNK, and p38 in LPS-induced RAW 264.7 cells. These findings indicate that CG exhibited potent anti-inflammatory activity through the NF-kappa B and MAPK signal pathway, and as a potential therapeutic agent against inflammatory diseases.