Recent developments in electrochemical detection for microchip capillary electrophoresis

被引：221

作者：

Vandaveer, WR ^{[1
]}

Pasas-Farmer, SA ^{[1
]}

Fischer, DJ ^{[1
]}

Frankenfeld, CN ^{[1
]}

Lunte, SM ^{[1
]}

机构：

[1] Univ Kansas, Dept Pharmaceut Chem, Lawrence, KS 66047 USA

来源：

ELECTROPHORESIS | 2004年 / 25卷 / 21-22期

关键词：

amperometric detection; conductimetric detection; microchip capillary electrophoresis; miniaturization; potentiometric detection; review;

D O I：

10.1002/elps.200406115

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Significant progress in the development of miniaturized microfluiclic systems has occurred since their inception over a decade ago. This is primarily due to the numerous advantages of microchip analysis, including the ability to analyze minute samples, speed of analysis, reduced cost and waste, and portability. This review focuses on recent developments in integrating electrochemical (EC) detection with microchip capillary electrophoresis (CE). These detection modes include amperometry, conductimetry, and potentiometry. EC detection is ideal for use with microchip CE systems because it can be easily miniaturized with no diminution in analytical performance. Advances in microchip format, electrode material and design, decoupling of the detector from the separation field, and integration of sample preparation, separation, and detection on-chip are discussed. Microchip CEEC applications for enzyme/immunoassays, clinical and environmental assays, as well as the detection of neurotransmitters are also described.

引用

页码：3528 / 3549

页数：22

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