Development of dimethyl sulfoxide biosensor using a mediator immobilized enzyme electrode

被引：12

作者：

Cheng, HC ^{[1
]}

Abo, M ^{[1
]}

Okubo, A ^{[1
]}

机构：

[1] Univ Tokyo, Dept Appl Biol Chem, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

ANALYST | 2003年 / 128卷 / 06期

关键词：

D O I：

10.1039/b212917e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A mediator immobilized dimethyl sulfoxide (DMSO) sensor using DMSO reductase (DMSO-R) was constructed. Methyl viologen (MV) was used as a mediator and immobilized on a glassy carbon (GC) electrode with Nafion polymer. DMSO-R from Rhodobacter sphaeroides f. sp. denitrificans was retained by a dialysis film on the modified GC electrode. The amperometric signal in response to DMSO was observed. The linear range of the calibration curve for DMSO was between 0 and 600 muM. The response time was within 100 s and the relative standard deviation was 4% at 200 muM DMSO (n = 4). To eliminate the background noise derived from oxygen in samples, the glucose oxidase-catalase retained DMSO sensor was also examined.

引用

页码：724 / 727

页数：4

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