Straightforward protein immobilization on Sylgard 184 PDMS microarray surface

被引：38

作者：

Heyries, Kevin A. ^{[1
]}

Marquette, Christophe A. ^{[1
]}

Blum, Loic J. ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS, Lab Genie Enzymat & Biomol, Inst Chim & Biochim Mol & Supramol,5246 ICBMS, F-69622 Villeurbanne, France

来源：

LANGMUIR | 2007年 / 23卷 / 08期

关键词：

D O I：

10.1021/la070018o

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this work, a straightforward technique for protein immobilization on Sylgard 184 is described. The method consists of a direct transfer of dried protein/salt solutions to the PDMS interface during the polymer curing. Such non-conventional treatment of proteins was found to have no major negative consequence on their integrity. The mechanisms of this direct immobilization were investigated using a lysine modified dextran molecule as a model. Clear experimental results suggested that both chemical bounding and molding effect were implicated. As a proof of concept study, three different proteins were immobilized on a single microarray (Arachis hypogaea lectin, rabbit IgG, and human IgG) and used as antigens for capture of chemiluminescent immunoassays. The proteins were shown to be easily recognized by their specific antibodies, giving antibody detection limits in the fmol range.

引用

页码：4523 / 4527

页数：5

共 48 条

[21] Surface biopassivation of replicated poly(dimethylsiloxane) microfluidic channels and application to heterogeneous immunoreaction with on-chip fluorescence detection [J].