Alcohol Biosensing by Polyamidoamine (PAMAM)/Cysteamine/Alcohol Oxidase-Modified Gold Electrode

被引：46

作者：

Akin, Mehriban ^{[1
]}

Yuksel, Merve ^{[1
]}

Geyik, Caner ^{[1
]}

Odaci, Dilek ^{[1
]}

Bluma, Arne ^{[2
]}

Hoepfner, Tim ^{[2
]}

Beutel, Sascha ^{[2
]}

Scheper, Thomas ^{[2
]}

Timur, Suna ^{[1
]}

机构：

[1] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova, Turkey

[2] Leibniz Univ Hannover, Inst Tech Chem, D-30167 Hannover, Germany

来源：

BIOTECHNOLOGY PROGRESS | 2010年 / 26卷 / 03期

关键词：

alcohol oxidase; electrochemical enzyme biosensor; dendrimer; self-assembled monolayers; AMPEROMETRIC BIOSENSOR; STARBURST DENDRIMERS; ENZYME ELECTRODE; GAS-CHROMATOGRAPHY; MELDOLAS BLUE; ETHANOL; IMMOBILIZATION; DEHYDROGENASE; STABILIZATION; SENSOR;

D O I：

10.1002/btpr.372

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A highly stable and sensitive amperometric alcohol biosensor was developed by immobilizing alcohol oxidase (A OX) through Polyamidoamine (PAMAM) dendrimers on a cysteamine-modified gold electrode surface. Ethanol determination is based on the consumption of dissolved oxygen content due to the enzymatic reaction. The decrease in oxygen level was monitored at -0.7 V vs. Ag/AgCl and correlated with ethanol concentration. Optimization of variables affecting the system was performed. The optimized ethanol biosensor showed a wide linearity from 0.025 to 1.0 mM with 100 s response time and detection limit of (LOD) 0.016 mM. In the characterization studies, besides linearity some parameters such as operational and storage stability, reproducibility, repeatability, and substrate specificity were studied in detail. Stability studies showed a good preservation of the bioanalytical properties of the sensor, 67% of its initial sensitivity was kept after 1 month storage at 4 degrees C. The analytical characteristics of the system were also evaluated for alcohol determination in flow injection analysis (FIA) mode. Finally, proposed biosensor was applied for ethanol analysis in various alcoholic beverage as well as offline monitoring of alcohol production through the yeast cultivation. (C) 2010 American Institute of Chemical Engineers Biotechnol. Prog., 26: 896-906, 2010

引用

页码：896 / 906

页数：11

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