DNA PROTOCOLS FOR TYPING FORENSIC BIOLOGICAL EVIDENCE - CHEMILUMINESCENT DETECTION FOR HUMAN DNA QUANTITATION AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM (RFLP) ANALYSES AND MANUAL TYPING OF POLYMERASE CHAIN-REACTION (PCR) AMPLIFIED POLYMORPHISMS

被引：49

作者：

BUDOWLE, B ^{[1
]}

BAECHTEL, FS ^{[1
]}

COMEY, CT ^{[1
]}

GIUSTI, AM ^{[1
]}

KLEVAN, L ^{[1
]}

机构：

[1] HELGERMAN COURT,GIBCO BRL,LIFE TECHNOL,GAITHERSBURG,MD

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 09期

关键词：

CHEMILUMINESCENCE; POLYMERASE CHAIN REACTION; MULTIPLEX AMPLIFICATION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; DNA QUANTITATION; SLOT BLOT; DNA EXTRACTION;

D O I：

10.1002/elps.11501601259

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods for identity testing are described that enable extraction of DNA from biological samples, determination of the quantity of human DNA, and genetic analyses of the materials using restriction fragment length polymorphism (RFLP) typing and/or amplified fragment length polymorphism (AMP-FLP) typing of PCR products. The salient features of the procedures are simplicity, manual typing, nonradioactive chemiluminescent assays or silver staining for detection, and low cost. Most application-oriented laboratories involved in forensic and/or paternity testing should be able to implement these procedures.

引用

页码：1559 / 1567

页数：9

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