Hydrolysis of rice bran oil using an immobilized lipase from Candida rugosa in isooctane

被引:13
作者
Murty, VR [1 ]
Bhat, J [1 ]
Muniswaran, PKA [1 ]
机构
[1] Deemed Univ, Dept Chem Engn, Manipal Inst Technol, Manipal Acad Higher Educ, Manipal 576104, India
关键词
immobilized lipase; isooctane; operational stability; packed bed reactor; rice bran oil;
D O I
10.1023/B:BILE.0000021956.33855.11
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The kinetics of enzymatic hydrolysis of rice bran oil in isooctane by immobilized Candida rugosa lipase in a batch reactor showed competitive inhibition by isooctane with a dissociation constant, K-I, of 0.92 M. Continuous hydrolysis of rice bran oil was performed in recycling, packed bed reactor with 4352 U of immobilized lipase; the optimum recycle ratio was 9 and the operational half-life was 360 h without isooctane but 288 h with 25% (v/v) isooctane in rice bran oil.
引用
收藏
页码:563 / 567
页数:5
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