Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: A homogeneous chemiluminescent assay for plasma choline

被引：11

作者：

Adamczyk, M ^{[1
]}

Brashear, RJ ^{[1
]}

Mattingly, PG ^{[1
]}

机构：

[1] Abbott Labs, Div Diagnost, Abbott Pk, IL 60064 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2006年 / 16卷 / 09期

关键词：

choline; chemiluminescence; homogeneous assay; acridinium-9-carboxamide;

D O I：

10.1016/j.bmcl.2006.01.114

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Hydrogen peroxide generated from the enzymatic oxidation of choline was detected using a chemiluminescent acridinium-9-carboxamide. The dose-response for choline (0-50 mu M) was established in buffer and was applicable to the quantification of choline in human plasma. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (1 mu L) and analysis time (<5 s per well). (C) 2006 Elsevier Ltd. All rights reserved.

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收藏

页码：2407 / 2410

页数：4

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