Expressed sequence tags from roots and nodule primordia of Lotus japonicus infected with Mesorhizobium loti

被引：16

作者：

Poulsen, C ^{[1
]}

Podenphant, L ^{[1
]}

机构：

[1] Univ Aarhus, Gene Express Lab, Dept Mol & Struct Biol, Aarhus C, Denmark

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2002年 / 15卷 / 04期

关键词：

legumes; root nodule development;

D O I：

10.1094/MPMI.2002.15.4.376

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Messenger RNA from young Lotus japonicus roots carrying root nodule primordia appearing after inoculation with Mesorhizobium loti bacteria were used to construct a cDNA expression library. Single-pass sequencing employing colony-polymerase chain reaction (PCR) and analysis of PCR products established a total of 2,397 new expressed sequence tags (ESTs). We have putatively identified 1,236 known and 484 hypothetical proteins coded by the corresponding mRNAs. The remaining cDNAs are unknown (316) or redundant overlapping cDNAs (361). We hope that this batch of ESTs will assist in the recognition of plant genes involved during development of nitrogen-fixing root nodules.

引用

页码：376 / 379

页数：4

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