A Molecular Peptide Beacon for the Ratiometric Sensing of Nucleic Acids

被引：140

作者：

Wu, Junchen ^{[2
,3
,5
]}

Zou, Ying ^{[1
]}

Li, Chunyan ^{[1
]}

Sicking, Wilhelm ^{[5
]}

Piantanida, Ivo ^{[4
]}

Yi, Tao ^{[1
]}

Schmuck, Carsten ^{[5
]}

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200438, Peoples R China

[2] E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China

[3] E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

[4] Rudjer Boskovic Inst, HR-10000 Zagreb, Croatia

[5] Univ Duisburg Essen, Inst Organ Chem, D-45117 Essen, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2012年 / 134卷 / 04期

基金：

美国国家科学基金会;

关键词：

CELL-PENETRATING PEPTIDES; FLUORESCENT COLOR READOUT; MINOR-GROOVE; DNA; BINDING; WATER; RECEPTOR; PROTEIN; DESIGN; BASE;

D O I：

10.1021/ja2103845

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A pyrene-functionalized cationic oligopeptide 1 efficiently binds to double-stranded DNA, as shown by different spectrophotochemical studies. Upon binding, the conformation of 1 changes from a folded to an extended form, which leads to a distinct change in the fluorescence properties. Thus, 1 functions as a molecular peptide beacon, and as it is easily taken up by cells, 1 can also be used for imaging of nucleic acids within cells.

引用

页码：1958 / 1961

页数：4

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