Plate-to-plate fluorous solid-phase extraction for solution-phase parallel synthesis

被引：27

作者：

Zhang, W ^{[1
]}

Lu, YM ^{[1
]}

Nagashima, T ^{[1
]}

机构：

[1] Univ Pittsburgh, Fluorous Technol Inc, Appl Res Ctr, Pittsburgh, PA 15238 USA

来源：

JOURNAL OF COMBINATORIAL CHEMISTRY | 2005年 / 7卷 / 06期

关键词：

D O I：

10.1021/cc050061z

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A commercially available Argonaut VacMaster-96 plate-to-plate solid-phase extraction (SPE) station equipped with 24 FluoroFlash cartridges is employed for parallel purification of fluorous reaction mixtures. Each cartridge charged with 3 g of fluorous silica gel has the capability to produce up to 100 mg of purified small molecules. The 24-well receiving plate has a standard footprint that can be directly concentrated in a Genevac vacuum centrifuge. Important issues such as sample loading, product cross-contamination, cartridge reuse, and reproducibility are investigated. The SPE system has been demonstrated in the purification of three small libraries that were produced involving amine scavenging reactions with fluorous isatoic anhydride, amide coupling reactions with 2-chloro-4,6-bis[(perfluorohexyl)propyloxyl-1,3,5-triazine (fluorous CDMT), and amide coupling reactions with a newly developed fluorous Mukaiyama condensation reagent.

引用

页码：893 / 897

页数：5

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