Robotic spotting of cDNA and oligonucleotide microarrays

被引：52

作者：

Auburn, RP

Kreil, DP

Meadows, LA

Fischer, B

Matilla, SS

Russell, S

机构：

[1] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England

[2] Univ Cambridge, Dept Phys, Cavendish Lab, Inference Grp, Cambridge CB3 0HE, England

来源：

TRENDS IN BIOTECHNOLOGY | 2005年 / 23卷 / 07期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/j.tibtech.2005.04.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA microarrays are a uniquely efficient method for simultaneously assessing the expression levels of thousands of genes. Owing to their flexibility and value, mechanically spotted microarrays remain the most popular platform. Here, we review recent technological advances with a focus on spotted arrays. Robotic spotting still poses numerous technical challenges. To reduce artefacts, many laboratories have recently investigated ways of improving the spotting process. We compare alternative options and discuss implications for next-generation systems. Together with modern approaches to data analysis, such developments bring greatly improved reliability to individual microarray experiments. Advancing towards the ultimate goal of delivering calibrated, truly quantitative gene-expression measurements on a genomic scale, microarray technology remains at the forefront of post-genomic systems biology.

引用

页码：374 / 379

页数：6

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