Biotinylated boronic acid fluorophore conjugates: Quencher elimination strategy for imaging and saccharide detection

被引：22

作者：

D'Hooge, Francois ^{[1
,2
]}

Elfeky, Souad A. ^{[1
,2
,3
]}

Flower, Stephen E. ^{[2
]}

Pascu, Sofia I. ^{[2
]}

Jenkins, A. Toby A. ^{[2
]}

van den Elsen, Jean M. H. ^{[4
]}

James, Tony D. ^{[2
]}

Fossey, John S. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands, England

[2] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

[3] Cairo Univ, Natl Inst Laser Enhanced Sci, Cairo, Egypt

[4] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England

来源：

RSC ADVANCES | 2012年 / 2卷 / 08期

基金：

英国工程与自然科学研究理事会;

关键词：

APPENDED BIPYRIDINIUM SALTS; MONOSACCHARIDE DETECTION; ENANTIOMERIC PURITY; SELECTIVE DETECTION; SIMPLE PROTOCOLS; SENSING SYSTEM; NMR ANALYSIS; VIOLOGEN; PURIFICATION; CHARGE;

D O I：

10.1039/c2ra00542e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A biotinylated boronic acid fluorophore conjugate bound to a diol-quencher via a boronic ester linkage demonstrated a fluorescence increase upon exposure to model saccharides. The sensing motif was attached to SuperAvidin microspheres where the sensing regime was imaged by fluorescence microscopy.

引用

页码：3274 / 3280

页数：7

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