Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A

被引：122

作者：

Yang, Cheng ^{[1
,2
]}

Lates, Vasilica ^{[3
]}

Prieto-Simon, Beatriz ^{[4
]}

Marty, Jean-Louis ^{[3
]}

Yang, Xiurong ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Elect Chem, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] Univ Perpignan, IMAGES EA 4218, F-66860 Perpignan, France

[4] Inst Bioengn Catalunya IBEC, Barcelona 08028, Spain

来源：

BIOSENSORS & BIOELECTRONICS | 2012年 / 32卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Ochratoxin A; Aptamer; G-quadruplex; DNAzyme; Hairpin; LINKED-IMMUNOSORBENT-ASSAY; G-QUADRUPLEX APTAMERS; SINGLE-STRANDED-DNA; MOLECULES; SELECTION; ENZYME; INVITRO; LIGANDS; BIND;

D O I：

10.1016/j.bios.2011.12.011

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We report an aptasensor for biosensing of Ochratoxin A (OTA) using aptamer-DNAzyme hairpin as biorecognition element. The structure of this engineered nucleic acid includes the horseradish peroxidase (HRP)-mimicking DNAzyme and the OTA specific aptamer sequences. A blocking tail captures a part of these sequences in the stem region of the hairpin. In the presence of OTA, the hairpin is opened due to the formation of the aptamer-analyte complex. As a result, self-assembly of the active HRP-mimicking DNAzyme occurs. The activity of this DNAzyme is linearly correlated with OTA concentration up to 10 nM, showing a limit of detection of 2.5 nM. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：208 / 212

页数：5

共 30 条

[21] Nucleic acid aptamers for clinical diagnosis: cell detection and molecular imaging [J].

Soontornworajit, Boonchoy ;

Wang, Yong .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 399 (04) :1591-1599

[22] Aptamer-DNAzyme Hairpins for Amplified Biosensing [J].

Teller, Carsten ;

Shimron, Simcha ;

Willner, Itamar .

ANALYTICAL CHEMISTRY, 2009, 81 (21) :9114-9119

[23] Analytical applications of aptamers [J].

Tombelli, S ;

Minunni, M ;

Mascini, A .

BIOSENSORS & BIOELECTRONICS, 2005, 20 (12) :2424-2434

[24] SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT - RNA LIGANDS TO BACTERIOPHAGE-T4 DNA-POLYMERASE [J].

TUERK, C ;

GOLD, L .

SCIENCE, 1990, 249 (4968) :505-510

[25] Biosensors and multiple mycotoxin analysis [J].

van der Gaag, B ;

Spath, S ;

Dietrich, H ;

Stigter, E ;

Boonzaaijer, G ;

van Osenbruggen, T ;

Koopal, K .

FOOD CONTROL, 2003, 14 (04) :251-254

[26]

Velasco-Garcia MN, 2009, GENE THER MOL BIOL, V13, P1

[27] Molecular Engineering of DNA: Molecular Beacons [J].

Wang, Kemin ;

Tang, Zhiwen ;

Yang, Chaoyong James ;

Kim, Youngmi ;

Fang, Xiaohong ;

Li, Wei ;

Wu, Yanrong ;

Medley, Colin D. ;

Cao, Zehui ;

Li, Jun ;

Colon, Patrick ;

Lin, Hui ;

Tan, Weihong .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (05) :856-870

[28] Aptamer-based colorimetric biosensing of Ochratoxin A using unmodified gold nanoparticles indicator [J].

Yang, Cheng ;

Wang, Yong ;

Marty, Jean-Louis ;

Yang, Xiurong .

BIOSENSORS & BIOELECTRONICS, 2011, 26 (05) :2724-2727

[29] Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection [J].

Yang, Xuan ;

Li, Tao ;

Li, Bingling ;

Wang, Erkang .

ANALYST, 2010, 135 (01) :71-75

[30] Development of a sensitive enzyme-linked immunosorbent assay for the determination of ochratoxin A [J].

Yu, FY ;

Chi, TF ;

Liu, BH ;

Su, CC .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (17) :6947-6953

← 1 2 3 →