Ultrasound permeabilizes CHO cells for the endonucleases AluI and benzon nuclease

被引：7

作者：

Johannes, C ^{[1
]}

Obe, G ^{[1
]}

机构：

[1] UNIV ESSEN GESAMTHSCH,DEPT GENET,D-45117 ESSEN,GERMANY

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1997年 / 374卷 / 02期

关键词：

chromosomal aberration; CHO cell; endonuclease; AluI; benzon nuclease; ultrasound; sonication;

D O I：

10.1016/S0027-5107(96)00238-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ultrasound permeabilizes Chinese hamster ovary (CHO) cells for the endonucleases AluI and benzon nuclease which leads to the induction of chromosomal aberrations by these enzymes. A few aberrant cells were observed when trypsinized cells or adherent cells were exposed to the enzymes in the absence of ultrasound. Our data show that sonication can he used to introduce endonucleases into CHO cells. We further demonstrate that few cells can internalize endonucleases without previous permeabilization.

引用

页码：245 / 251

页数：7

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