Quinohemoprotein alcohol dehydrogenase-based reagentless amperometric biosensor for ethanol monitoring during wine fermentation

被引:75
作者
Niculescu, M
Erichsen, T
Sukharev, V
Kerenyi, Z
Csöregi, E
Schuhmann, W
机构
[1] Lund Univ, Dept Biotechnol, S-22100 Lund, Sweden
[2] Ruhr Univ Bochum, D-44780 Bochum, Germany
[3] Moscow MV Lomonosov State Univ, Dept Chem, RU-119899 Moscow, Russia
[4] Minist Agr, Res Inst Viticulture & Enol, H-6001 Kecskemet, Hungary
关键词
alcohol dehydrogenase; ethanol; biosensor; fermentation; on-line process control; redox polymer; sequential-injection analysis;
D O I
10.1016/S0003-2670(02)00344-6
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This paper describes the development and optimization of an amperometric biosensor for monitoring ethanol in beverages. The biosensor is constructed by cross-linking a quinoprotein alcohol dehydrogenase (QH-ADH) to an Os-complex-modified poly(vinylimidazole) redox polymer using poly(ethylene glycol) diglycidyl ether. The optimum biosensor configuration was evaluated by changing the ratio between enzyme, redox polymer, and cross-linker using conventional graphite rods as basis electrodes. The optimized sensor showed a sensitivity of 0.336+/-0.025 A M-1 cm(2) for ethanol and a detection limit (calculated as three times the signal-to-noise ratio) of 1 muM. This biosensor configuration was further evaluated in a conventional flow-injection system and the applicability for the determination of ethanol in diverse wine samples could be successfully demonstrated. Adaptation of this sensor configuration to screen-printed (SP) electrodes allowed their integration into an automated sequential-injection analyzer and the successful on-line monitoring of ethanol during wine fermentation processes. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:39 / 51
页数:13
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