In vivo gated 4D imaging of the embryonic heart using optical coherence tomography

被引：74

作者：

Jenkins, Michael W.

Chughtai, Osman Q.

Basavanhally, Ajay N.

Watanabe, Michiko

Rollins, Andrew M.

机构：

[1] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Sch Med, Dept Pediat, Cleveland, OH 44106 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2007年 / 12卷 / 03期

关键词：

optical coherence tomography; embryonic hearts; medical imaging; avian embryos; gated imaging;

D O I：

10.1117/1.2747208

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate the first in vivo gated 4D images of avian embryonic hearts by use of optical coherence tomography (OCT). We present a gated 4D dataset of an in vivo beating quail heart consisting of similar to 864,000 A-scans accumulated over multiple heartbeats. Generation of a gating trigger from a laser Doppler velocimetry (LDV) signal, collected from an outlying vitelline vessel, enabled us to gate image acquisition to the cardiac cycle. To fully characterize the genesis and mechanisms of cardiac defects, a tool capable of assessing structure and function simultaneously at early stages of development is needed, and gated OCT has the capability to become such a tool. (C) 2007 society of Photo-Optical Instrumentation Engineers.

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