Immunoassays in microfluidic systems

被引：285

作者：

Ng, Alphonsus H. C. ^{[2
,3
]}

Uddayasankar, Uvaraj ^{[3
]}

Wheeler, Aaron R. ^{[1
,2
,3
]}

机构：

[1] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada

[2] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada

[3] Terrence Donnelly Ctr Cellular & Biomol Res, Toronto, ON M5S 3E1, Canada

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 397卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Microfluidics/microfabrication; Immunoassays/ELISA; Biosensors; Bioanalytical methods; Fluid handling/automation multiplex analysis; LINKED-IMMUNOSORBENT-ASSAY; C-REACTIVE PROTEIN; ON-A-CHIP; SIMULTANEOUS MULTIPLE IMMUNOASSAYS; ASSISTED DENDRITIC AMPLIFICATION; LATEX IMMUNOAGGLUTINATION ASSAY; BEAD-BASED IMMUNOASSAY; HUMAN SERUM; LABEL-FREE; CAPILLARY-ELECTROPHORESIS;

D O I：

10.1007/s00216-010-3678-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunoassays have greatly benefited from miniaturization in microfluidic systems. This review, which summarizes developments in microfluidics-based immunoassays since 2000, includes four sections, focusing on the configurations of immunoassays that have been implemented in microfluidics, the main fluid handling modalities that have been used for microfluidic immunoassays, multiplexed immunoassays in microfluidic platforms, and the emergence of label-free detection techniques. The field of microfluidic immunoassays is continuously improving and has great promise for the future.

引用

页码：991 / 1007

页数：17

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