PARTIAL-PURIFICATION AND PROPERTIES OF A CIS-3 - TRANS-2-ENAL ISOMERASE FROM CUCUMBER FRUIT

被引：45

作者：

PHILLIPS, DR

MATTHEW, JA

REYNOLDS, J

FENWICK, GR

机构：

[1] Agricultural Research Council, Food Research Institute, Norwich, NR4 7UA, Colney Lane

来源：

PHYTOCHEMISTRY | 1979年 / 18卷 / 03期

关键词：

cis-3: trans-2-enal isomerase; cleavage enzyme; cucumber; Cucumis sativus; Cucurbitaceae; fatty acid hydroperoxide; flavour; volatile aldehydes;

D O I：

10.1016/S0031-9422(00)81874-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme, which catalyses the isomerisation of cis-3-enals to trans-2-enals, has been partially purified from cucumber fruit. The isomerase activity has been resolved from significant contamination by the related activities, lipoxygenase and hydroperoxide cleavage enzymes. An examination of the substrate specificity of the isomerase enzyme showed it to be specific for the cis-3-enals. The most efficient isomerisation was achieved with cis-3-hexenal and cis-3-nonenal which are, physiologically, the two most significant substrates. The trans-3-enal and cis-3-enol were not suitable substrates for the enzyme. © 1979.

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页码：401 / 404

页数：4

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