NOVEL SOLID SUPPORTS FOR THE PREPARATION OF 3'-DERIVATIZED OLIGONUCLEOTIDES - INTRODUCTION OF 3'-ALKYLPHOSPHATE TETHER GROUPS BEARING AMINO, CARBOXY, CARBOXAMIDO, AND MERCAPTO FUNCTIONALITIES

被引：38

作者：

HOVINEN, J ^{[1
]}

GUZAEV, A ^{[1
]}

AZHAYEV, A ^{[1
]}

LONNBERG, H ^{[1
]}

机构：

[1] UNIV TURKU,DEPT CHEM,SF-20500 TURKU,FINLAND

来源：

TETRAHEDRON | 1994年 / 50卷 / 24期

关键词：

D O I：

10.1016/S0040-4020(01)85244-3

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Syntheses of 5 non-nucleosidic solid supports (1-5) that enable preparation of oligonucleotides bearing a carboxy,-amino,-carboxamido,-or mercaptoalkyl spacer arm at their 3'-terminus are described. They all contain an ester bond of moderate susceptibility toward nucleophiles. Upon the completion of oligonucleotide chain assembly, this bond may be cleaved by a variety of nucleophiles. These release the oligonucleotide from the support and simultaneously introduce the desired functionality. Differences in the reactivity between the supports prepared are discussed.

引用

页码：7203 / 7218

页数：16

共 35 条

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