RIBOFLAVIN-SENSITIZED PHOTODYNAMIC UV SPECTROPHOTOMETRY FOR ASCORBIC-ACID ASSAY IN BEVERAGES

被引：18

作者：

JUNG, MY ^{[1
]}

KIM, SK ^{[1
]}

KIM, SY ^{[1
]}

机构：

[1] CHUNGNAM NATL UNIV,DEPT FOOD SCI & TECHNOL,TAEJON,SOUTH KOREA

来源：

JOURNAL OF FOOD SCIENCE | 1995年 / 60卷 / 02期

关键词：

ASCORBIC ACID; PHOTODYNAMIC ASSAY; RIBOFLAVIN SENSITIZED; FRUIT JUICES; SPORTS DRINK;

D O I：

10.1111/j.1365-2621.1995.tb05673.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

photodynamic ultraviolet spectrophotometric assay of various commercial beverages was carried out. The maximum concentration of ascorbic acid for obtaining a background correction (15 min illumination and pH 7.5) was 21 mu g/mL ascorbic acid in the testing solution. The upper limit of the measurement range for a straight line in the calibration graph of standard ascorbic acid was 10 mu g/mL, (working range 0-10 mu g/mL). The results in repeatability of the method for ascorbic acid contents in commercial fruit juices and soft-drinks showed a maximum of 4% relative standard deviation. Recovery tests with known amounts of added ascorbic acid in different fruit juices and sports drink showed the recovery of added ascorbic acid was 97.5-102.3%. Indophenol, iodine and/or HPLC methods were used in parallel to ascertain the reliability of the proposed method. This type assay could be successfully applied to many commercial beverages for determination of ascorbic acid with good accuracy, precision and reliability.

引用

页码：360 / &

共 23 条

[1] ASCORBIC-ACID AS A SCAVENGER OF SINGLET OXYGEN [J].

BODANNES, RS ;

CHAN, PC .

FEBS LETTERS, 1979, 105 (02) :195-196

[2] ASCORBIC-ACID AND DEHYDROASCORBIC ACID CONTENT OF TROPICAL ROOT CROPS FROM THE SOUTH-PACIFIC [J].

BRADBURY, JH ;

SINGH, U .

JOURNAL OF FOOD SCIENCE, 1986, 51 (04) :975-&

[3]

BRADBURY JH, 1986, J FOOD SCI, V51, P987

[4] RAPID ENZYMATIC METHOD FOR VITAMIN-C ASSAY IN FRUITS AND VEGETABLES USING PEROXIDASE [J].

CASELLA, L ;

GULLOTTI, M ;

MARCHESINI, A ;

PETRARULO, M .

JOURNAL OF FOOD SCIENCE, 1989, 54 (02) :374-&

[5] L-ASCORBIC-ACID QUENCHING OF SINGLET DELTA MOLECULAR-OXYGEN IN AQUEOUS-MEDIA - GENERALIZED ANTIOXIDANT PROPERTY OF VITAMIN-C [J].

CHOU, PT ;

KHAN, AU .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 115 (03) :932-937

[6] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF ASCORBIC-ACID, ERYTHORBIC ACID, DEHYDROASCORBIC ACID, DEHYDROERYTHORBIC ACID, DIKETOGULONIC ACID, AND DIKETOGLUCONIC ACID [J].

DONER, LW ;

HICKS, KB .

ANALYTICAL BIOCHEMISTRY, 1981, 115 (01) :225-230

[7] DETERMINATION METHOD FOR L-ASCORBIC-ACID IN FOODS WITH IMMOBILIZED ASCORBATE OXIDASE [J].

ESAKA, M ;

SUZUKI, K ;

KUBOTA, K .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1985, 49 (10) :2955-2960

[8] EFFECTS OF CHLOROPHYLL AND BETA-CAROTENE ON THE OXIDATION STABILITY OF OLIVE OIL [J].

FAKOURELIS, N ;

LEE, EC ;

MIN, DB .

JOURNAL OF FOOD SCIENCE, 1987, 52 (01) :234-235

[9] DETERMINATION OF ASCORBIC-ACID IN SOFT DRINKS AND FRUIT JUICES .1. BACKGROUND CORRECTION FOR DIRECT ULTRAVIOLET SPECTROPHOTOMETRY [J].

FUNG, YS ;

LUK, SF .

ANALYST, 1985, 110 (02) :201-204

[10] DETERMINATION OF ASCORBIC-ACID IN SOFT DRINKS AND FRUIT JUICES .2. APPLICABILITY OF DIRECT ULTRAVIOLET SPECTROPHOTOMETRY WITH BACKGROUND CORRECTION [J].

FUNG, YS ;

LUK, SF .

ANALYST, 1985, 110 (12) :1439-1441

← 1 2 3 →