Homogeneous chemiluminescent assays for free choline in human plasma and whole blood

被引：16

作者：

Adamczyk, Maciej ^{[1
]}

Brashear, R. Jeffrey ^{[1
]}

Mattingly, Phillip G. ^{[1
]}

Tsatsos, Panagiota H. ^{[1
]}

机构：

[1] Abbott Labs, Div Diagnost, Abbott Pk, IL 60064 USA

来源：

ANALYTICA CHIMICA ACTA | 2006年 / 579卷 / 01期

关键词：

chemiluminescence; choline analysis; liquid chromatography-electrospray ionization tandem mass spectrometry correlation; plasma; whole blood;

D O I：

10.1016/j.aca.2006.07.020

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Choline was oxidized in the presence of choline oxidase and the hydrogen peroxide generated was detected using a chemiluminescent acridinium-9-carboxamide. The dose response for choline (0-150 mu M) was established in buffer and was validated for the quantification of choline in human plasma and whole blood. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (4 mu L) and short analysis time (< 5 s per well). The new assay(s) correlated well (R > 0.98, plasma; R > 0.97, whole blood) with LC-MS/MS. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：61 / 67

页数：7

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